Changes in root endogenous hormone levels and rhizosphere fungi diversity in alfalfa under drought stress

To explain the relationship between rhizosphere fungi diversity and community composition of alfalfa under drought stress endogenous hormones in alfalfa, we used sand culture to investigate performance Medicago sativa ‘Qingshui’ (or QS), M. ‘Longdong’ LD), varia ‘Gongnong No. 4’ GN) with contrasting different degrees tolerance. After 7 days treatment, underwent significant changes, found Ascomycota Basidiomycota were dominant groups all treatments, indicating that drought-stressed environments play a crucial role shaping their communities. The results showed effects on α-diversity, Chao1, Pielou-e, Shannon Simpson rhizospheric fungi. index was highest for severe LD, while Chao1 moderate QS GN. indices respective controls. In addition, study had impact hormone concentrations roots. As increased, levels gibberellin (GA3) zeatin (ZA), as well GA3/abscisic acid (ABA) ratio, initially increased then decreased, observed stress, indole-3-acetic (IAA), IAA/ABA, (IAA + GA3 ZA) /ABA decreased lowest ABA content roots stress. correlation (12.8%) GA3/ABA (12.7%) identified driving factors